Enzyme Lab Report- Jacob

Introduction:

  Enzymes are biological molecules that act as a catalyst with three characteristics. Enzyme increase the rate of reactions. Enzymes act with reactant called a substrate to produce a product.

Purpose:

 This is an experiment where we test the enzymes and how they are impacted by temperature changes.

HYPOTHESIS:

"If the temperature of water affects the production rate of enzymes,
then once the temperature of the water/substance has reached around room
temperature, the production rate will start to slow down.

Independent Variable:

Temperature (Celsius)

Dependent Variable:

the enzyme’s rate of reaction

Controlled Variables:

Volume of the extract

Justification of hypothesis:

Since enzymes work best at normal human body temperature, they could end up declining.

Materials:

-Test tubes                           -Ruler

-Oak grass                         -Beakers

-Hydrogen Peroxide          -Stopwatch

-Water

-Ice

PROCEDURE:

1. Collect and Set up the materials at a lab station with the ice or hot
   water baths that are Room temperature (20 degrees Celsius), Cold (0
   degrees Celsius), and hot (50 degrees Celsius)
2. Collect the oak grass and it with a mortar and pestle until it reaches a watery substance
3. Tape the ruler onto the test tubes.
4. To create
   the extract for this experiment, you need to take the 2 mL of the
   oak grass substance and mix it with a bit of water  into a test tube.
5. To heat up/cool down the extract, add it to the bath until it has reached the temperature you want it to be at.
6. When it is done add ½ mL of the per-oxidase to the test tubes
7. Measure
   how many bubbles appear once the reaction starts by timing how tall the
   bubbles get every 10 seconds (for a total of 60 seconds) and the height
   of the bubbles when it has reached its peak.
8. Repeat the process for remaining test tubes.
9. Collect data and analyze results.

Summary:

We started with the materials and slowly made our way to making test tubes
with different liquids. Once they were ready we measured the bubbles
and used the stopwatch to record their size every ten seconds. Our
results were then recorded on a table on a piece of paper. When we
finished testing every single test tube we came up with our final
results.

DATA AND RESULTS:

Cold- 0 degrees Celsius

Time:              Height:

0 sec             0 mm

10 sec           2 mm

20 sec          4 mm

30 sec          7 mm

40 sec         10 mm

50 sec         12 mm

60 sec         14 mm

Room Temperature- 20 degrees Celsius

Time: Height:

0 sec 0 mm

10 sec 30 mm

20 sec 35 mm

30 sec 40 mm

40 sec 45 mm

50 sec 49 mm

60 sec 50 mm

Hot- 50 degrees Celsius

Time: Height:

0 sec 0 mm

10 sec 19 mm

20 sec 25 mm

30 sec 33 mm

40 sec 40 mm

50 sec 42 mm

60 sec 45 mm

CONCLUSION:

Our hypothesis was wrong, our room temperature measurements increased every ten seconds. we thought it was going to change