Enzyme Lab Report- Sayra

Hypothesis:

 If the temperature of water affects the production rate of enzymes, then once the temperature of the water/substance has reached around room temperature ,the production rate will start to slow down.

Independent Variable:

Temperature (Celsius)

Dependent Variable:

the enzyme’s rate of reaction

Controlled Variables:

Volume of the extract

Justification of hypothesis:

Since enzymes work best at normal human body temperature, they could end up declining.

Materials:

-Testtubes                 -Ruler

-Oakgrass -Beakers

-Hydrogen Peroxide -Stopwatch

-Water

-Ice

Procedure:

1. 1) Collect and Set up the materials at a lab station with the ice or hot water baths that are Room temperature (20 degrees Celsius), Cold (0 degrees Celsius), and hot (50 degrees celsius)
2. Collect the oakgrass and it with a mortar and pestle until it reaches a watery substance
3. Tape the ruler onto the test tubes.
4. To create the extract for this experiment, you need to take the 2 mL of the oakgrass substance and mix it with a bit of water  into a test tube.
5. To heat up/cool down the extract, add it to the bath until it has reached the temperature you want it to be at.
6. When it is done add ½ mL of the peroxidase to the test tubes
7. Measure how many bubbles appear once the reaction starts by timing how tall the bubbles get every 10 seconds (for a total of 60 seconds) and the height of the bubbles when it has reached its peak.
8. Repeat the process for remaining test tubes.
9. Collect data and analyze results.

Summary:

We started with the materials and slowly made our way to making test tubes with different liquids. Once they were ready we measured the bubbles and used the stopwatch to record their size every ten seconds. Our results were then recorded on a table on a piece of paper. When we finished testing every single test tube we came up with our final results.

Data and Results:

Cold- 0 degrees Celsius

Time:              Height:

0 sec 0 mm

10 sec 2 mm

20 sec 4 mm

30 sec 7mm

40 sec 10mm

50 sec 12mm

60 sec 14mm

Room Temperature- 20 degrees Celsius

Time: Height:

0 sec 0 mm

10 sec 30 mm

20 sec 35mm

30 sec 40mm

40 sec 45mm

50 sec 49mm

60 sec 50mm

Hot- 50 degrees Celsius

Time: Height:

0 sec 0mm

10 sec 19mm

20 sec 25mm

30 sec 33mm

40 sec 40mm

50 sec 42mm

60 sec 45 mm

-I did not understand how to make a chart on here.

Conclusion:

Our hypothesis was wrong, our room temperature measurements increased every ten seconds.