Enzyme Lab Report- Jenny

Introduction:
       It is said that enzymes work best at normal room temperature, so our group decided to test this. After much research, we discovered that the enzymes work best under normal room temperature, which is around 20 degrees Celsius.
Hypothesis:

       If the temperature of water affects the production rate of enzymes, then once the temperature of the water/substance has reached around normal air temperature (20 degrees Celsius), the production rate will start to decline. 

Independent Variable: 

       Temperature in Celsius

Dependent Variable:

       Rate of reaction

Controlled Variable:

       The protein source and (hydrogen peroxide) and the type of water

Justification of Hypothesis:

       I wanted to know at what point the productivity of the enzymes starts to decline, and enzymes work best at normal human body temperature which could mean that this could be the point where it starts to decline.

Materials: 

digital scale one handful of freshly picked bindweed vines with leaves (5-10 grams)mortar and pestle distilled water 3 one hundred liter beakers 2 ml. syringe hydrogen peroxide paper towel 8 glass test tubes, test tube rack, small plastic ruler, safety goggles ice 3 large plastic beaker (ice bath, hot water, body temperature water) hot water thermometers
Procedure:

We are going to test the effects that temperature has on the production rate of enzymes. We are going to have 3 different beakers filled with 20, 50, 40, and 3 degrees Celsius. We are going to test the rate of reaction in these three temperatures.

Steps:

1. Collect and Set up the materials at a lab station with the ice/hot water baths (20, 50, 40, and 3 degrees Celsius)
2. Collect the bindweed leaves and grind them up with a mortar and pestle until it reaches a watery substance 
3. Tape the ruler onto the test tubes.
4. To create the extract for this experiment, you need to take the 35 grams of the bindweed and add it to 55 mL of water.
5. Filter the substance through a paper towel and into a beaker. You will then have 60 mL extract that looks like green water. 
6. Add 2 mL of the extract into each test tube. (you don’t need a lot of the extract in each tube, but you make enough just in case.
7. To heat up/cool down the extract, add it to the bath until it has reached the temperature you want it to be at. 
8. Add the bindweed to the test tube.
9. Add 3 mL peroxidase to the four test tubes. 
10. Measure how many bubbles appear once the reaction  starts by timing how tall the bubbles get every 5 seconds (for a total of 30 seconds) and the height of the bubbles when it has reached its peak. 
11. Repeat the process for remaining test tubes. 
12. Collect data and analyze results.

Data and Results:

(This section should include at least one graph!) *

Time in seconds       Height in centimeters

Control (20 degrees Celsius)- Time: Height

0: 6.1

5: 6.1

10: 6.2

15: 6.3

20: 6.6

25: 6.8

30: 6.8

Temperature #1- hot (50 degrees Celsius)- Time: Height

0: 6.1

5: 6.1

10: 6.2

15: 6.2

20: 6.3

25: 6.3

30: 6.4

Temperature #2- medium (40 degrees Celsius)- Time: Height

0:6.1

5: 6.1

10: 6.2

15:6.4

20:6.5

25: 6.5

30: 6.5

Temperature #4- Cold (3 degrees Celsius) Time: Height

0:6.1

5: 6.1

10: 6.1

15: 6.1

20: 6.1

25: 6.2

30: 6.2
*I couldn't figure out how to make a graph on this, but I am going to keep trying to get one.

Conclusions:

Our hypothesis was confirmed as true, because after the temperature reached normal body temperature, the production rate started to decline.